Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic BacteriaEntry ID: ASAC_2547
Abstract: Metadata record for data from ASAC Project 2547
See the link below for public details on this project.
Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate ... bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this.
This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]).
The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.
Data Set Citation
Dataset Originator/Creator: Codd, R.
Dataset Title: Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria
Dataset Series Name: CAASM Metadata
Dataset Publisher: Australian Antarctic Data Centre
Version: 1Online Resource: https://data.aad.gov.au/metadata/records/ASAC_2547
Start Date: 2004-07-01Stop Date: 2005-07-31
Quality The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012.
Access Constraints Copies of the publications, plus a list of accession numbers are available for download from the provided URL to AAD staff only.
Use Constraints This data set conforms to the CCBY Attribution License
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547 when using these data.
Data Set Progress
Distribution Media: HTTP
Distribution Size: 980 kb
Distribution Format: PDF
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Phone: +61 2 9351 6738
Fax: +61 2 9351 3868
Email: rachel.codd at sydney.edu.au
University of Sydney School of Medical Sciences
Province or State: New South Wales
Packchung, A.A.H., Simpson, P.J.L., Codd, R. (2006), Life on Earth. Extremophiles Continue to Move the Goal Posts, Environ. Chem., 3, 77-93
Packchung, A.A.H., Soe, C.Z., Codd, R. (2008), Studies of Iron-Uptake Mechanisms in Two Bacterial Species of the Shewanella Genus Adapted to Middle-Range (Shewanella putrefaciens) or Antarctic (Shewanella gelidimarina) Temperatures, Chemistry and Biodiversity, 5, 2113-2123
Simpson, P.J.L., Richardson, D.J., Codd, R. (2010), The periplasmic nitrate reductase in Shewanella: the resolution, distribution and functional implications of two NAP isoforms, NapEDABC and NapDAGHB, Microbiology, 156, 302-312
simpson, P.J.L., McKinzie, A.A., Codd, R. (2010), Resolution of two native monomeric 90 kDa nitrate reductase active proteins from Shewanella gelidimarina and the sequence of two napA genes, Biochemical and Biophysical research Communications, 398, 13-18
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2005-03-02
Last DIF Revision Date: 2019-04-08