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Data for manuscript 'Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity'
Entry ID: AAS_4026_Productivity_PAM_Phytoplankton_Bacteria


Summary
Abstract: This data set was collected from a ocean acidification minicosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes:
- description of methods for all data collection and analyses.
- marine microbial community data; Chlorophyll a concentration, particulate organic matter concentration (carbon and nitrogen), bacterial cell abundance.
- phytoplankton primary productivity data; 14C-sodium bicarbonate incorporation raw data (decays per minute: DPM) and modelled productivity from photosynthesis versus irradiance (PE) curves, O2-evolution derived net community productivity, respiration, and gross primary productivity.
- phytoplankton photophysiology data; community photosynthetic efficiency from PAM measurements (maximum quantum yield of PSII: Fv/Fm), PAM steady state light curve data and derived non-photochemical quenching of Chl a fluorescence (NPQ), relative electron transport rates (rETR), and effective quantum yield of PSII (delta F/Fm').
- phytoplankton carbon concentrating mechanism (CCM) data; maximum quantum yield of PSII (Fv/Fm) and effective quantum yield of PSII (?F/Fm') from PAM measurements on size-fractionated phytoplankton samples (less than 10 microns and greater than 10 microns cells) exposed to; ethoxzolamide (EZA) which inhibits both intracellular carbonic anhydrase (iCA) and extracellular carbonic anhydrase (eCA), acetazolamide (AZA), which blocks eCA only, and a control (no inhibitor) sample.
- bacterial productivity data; 14C-Leucine incorporation raw data (decays per minute: DPM) and calculated productivity.


Purpose: One community-level study has been performed in Antarctic waters on a natural coastal marine microbial community at Davis Station, Antarctica during the 2008/09 summer season. This study in Prydz Bay, East Antarctica reported declines in primary and bacterial productivity when CO2 concentration exceeded 780 micro atm. A shift in community composition was also observed, with increased abundance of small picoeukaryotes and a decline in large diatoms.

Based on the results of this previous study, a natural community of Antarctic marine microbes from Prydz Bay, East Antarctica were exposed to a range of CO2 concentrations in 650 l minicosms to simulate possible future ocean conditions up to the year ?2200. Productivity was examined at CO2 concentrations between 343 to 1641 ?atm to determine whether tipping points existed, above which CO2 concentration reduced rates of primary productivity, bacterial productivity, and the accumulation of particulate organic matter (POM). Photophysiological assays were also performed to assess whether possible mechanisms driving changes in the phytoplankton community could be determined.

Related URL
Link: View Related Information
Description: Citation reference for this metadata record and dataset.


Link: View Related Information
Description: Download the data


Link: View Related Information
Description: Public information for AAS project AAS_4026

Parent DIF
This description is a member of a collection. The collection is described in
AAS_4026_Ocean_Acidification_Marine_Microbes_Parent


Geographic Coverage
 N: -68.57644 S: -68.57644  E: 77.95898  W: 77.95898

Data Set Citation
Dataset Creator: Deppeler, S.L., Petrou, K., Schulz, K., Davidson, A.T., McKinlay, J., Pearce, I. and Westwood, K.J.
Dataset Title: Data for manuscript 'Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity'
Dataset Series Name: CAASM Metadata
Dataset Release Date: 2017-11-30
Dataset Publisher: Australian Antarctic Data Centre
Online Resource: https://data.aad.gov.au/aadc/metadata/metadata_redirect.cfm?md=/AMD...


Temporal Coverage
Start Date: 2014-11-19
Stop Date: 2014-12-08


Location Keywords
GEOGRAPHIC REGION > POLAR
CONTINENT > ANTARCTICA > Davis Station
OCEAN > SOUTHERN OCEAN


Data Resolution
Temporal Resolution: 0 Day(s)


Science Keywords
OCEANS >OCEAN CHEMISTRY >CARBON DIOXIDE    [Definition]
BIOSPHERE >ECOSYSTEMS >AQUATIC ECOSYSTEMS >PLANKTON >PHYTOPLANKTON    [Definition]
BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]
OCEANS >OCEAN CHEMISTRY >ORGANIC MATTER    [Definition]


Platform
LABORATORY    [Information]
FIELD INVESTIGATION    [Information]


Instrument
HPLC >High-Performance Liquid Chromatograph    [Information]
LBLSC >Low Background Liquid Scintillation Counter
FLOW CYTOMETRY    [Information]
CHNS/O ELEMENTAL ANALYZERS >Carbon, Hydrogen, Nitrogen Elemental Analyzers    [Information]
PAM >PORTABLE FLUORESCENCE ANALYZERS    [Information]
LICOR QUANTUM SENSOR    [Information]


Quality
The minicosm experimental design measured the microbial community growth in six unreplicated fCO2 treatments. Therefore, sub-samples from each minicosm were within-treatment pseudoreplicates and thus, results of statistical analysis must be interpreted conservatively.


Access Constraints
This data set is publicly available for download from the provided URL.


Use Constraints
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026... when using these data.


Keywords
ocean acidification
primary productivity
bacterial productivity
photophysiology
oxygen sensor spots


Data Set Progress
COMPLETE


Originating Center
Australian Antarctic Division


Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Distribution
Distribution_Media: HTTP
Distribution_Size: 850 kb
Distribution_Format: Excel
Fees: Free


Personnel
STACY LOUISE DEPPELER
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Email: stacy.deppeler at utas.edu.au
Email: stacy.deppeler at aad.gov.au
Contact Address:
Institute for Marine and Antarctic Studies
20 Castray Esplanade
City: Battery Point
Province or State: Tasmania
Postal Code: 7000
Country: Australia


KATHERINA PETROU
Role: INVESTIGATOR
Email: Katherina.Petrou at uts.edu.au
Contact Address:
University of Technology, Sydney
City: Sydney
Province or State: New South Wales
Country: Australia


KAI SCHULZ
Role: INVESTIGATOR
Email: kai.schulz at scu.edu.au
Contact Address:
Centre for Coastal Biogeochemistry
Southern Cross University
Military Rd
City: East Lismore
Province or State: New South Wales
Postal Code: 2480
Country: Australia


ANDREW TIMOTHY DAVIDSON
Role: INVESTIGATOR
Phone: +61 3 6232 3444
Fax: +61 6232 3351
Email: andrew.davidson at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


JOHN MCKINLAY
Role: INVESTIGATOR
Email: john.mckinlay at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


IMOJEN PEARCE
Role: INVESTIGATOR
Phone: +61 3 6232 3155
Email: imojen_pea at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


KAREN JILLIAN WESTWOOD
Role: INVESTIGATOR
Phone: +61 3 6232 3156
Email: karen.westwood at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


Publications/References
Stacy Deppeler, Katherina Petrou, Kai Schulz, Karen Westwood, Imojen Pearce, John McKinlay, and Andrew Davidson (2017), Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity, Biogeociences, submitted
Extended Metadata Properties
(Click to view more)

Creation and Review Dates
DIF Creation Date: 2017-08-21
Last DIF Revision Date: 2018-07-10



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