Abstract:
Antarctic sediments and sea-ice are important regulators in global biogeochemical and atmospheric cycles. These ecosystems contain a diverse range of bacteria whose biogeochemical roles remains largely unknown and which inhabit what are continually low temperature habitats. An integrated molecular and chemical approach will be used to investigate the coupling of microbial biogeochemical processes ... with community structure and cold adaptation within coastal Antarctic marine sediments and within sea-ice. Overall the project expects to make an important contribution to our understanding of biological processes within low temperature habitats.
DATA SET ORGANISATION:
The dataset is organised on the basis of publication and is organised on the basis of the following sections:
1. SEDIMENT SAMPLES and ISOLATES
Samples collected are described in terms of location, type and where data were obtained chemical features. The designation, source, media used for cultivation and isolation and availability of sediment and other related isolates are provided. Samples included are from the following locations: Clear Lake, Pendant Lake, Scale Lake, Ace Lake, Burton Lake, Ekho Lake, Organic Lake, Deep lake and Taynaya Bay (Burke Basin), Vestfold Hills region; and the Mertz Glacier Polynya region.
2. BIOMASS and ENZYME ACTIVITY DATA
Biomass, numbers and extracellular enzyme activity data are provided for Bacteria and Archaea populations from Mertz Glacier Polynya shelf sediments.
3. FATTY ACID and TETRAETHER LIPID DATA
Phospholipid and tetraether lipid data are provided for Mertz Glacier Polynya shelf sediments. Whole cell fatty acid data are provided for various bacterial isolates described officially as new genera or species.
4. RNA HYBRIDISATION DATA
RNA hybridisation data for Mertz Glacier Polynya sediment samples is provided, including data for oligonucleotide probes specifc for total Bacteria, Archaea, the Desulfosarcina group (class Deltaproteobacteria, sulfate reducing bacterial clade), phylum Planctomycetes, phylum Bacteroidetes (Cytophaga-Flavobacterium-Bacteroides), class Gammaproteobacteria, sulfur-oxidizing and related bacteria (a subset of class Gammaproteobacteria) and Eukaryota.
5. PHYLOGENETIC DATA
16S rRNA gene sequence data are indicated including aligned datasets for three clone libraries derived from the Mertz Glacier Polynya including GenBank accession numbers. Sequence accession numbers are provided for Vestfold Hills lake sediment samples. In addition GenBank numbers are provided for denaturing gradient gel electrophoresis band sequence data from Mertz Glacier Polynya shelf sediment. Other forms of this DGGE data (banding profile analysis) are available in reference Bowman et al. 2003 (AAD ref 10971).
School of Agricultural Science
University of Tasmania
Private Bag 54
City:
Hobart
Province or State:
Tasmania
Postal Code:
7001
Country:
Australia
Publications/References
1. Bowman, J.P., McCammon, S.A., Rea, S.M., McMeekin, T.A. (2000) The microbial composition of three limnologically disparate hypersaline Antarctic lakes FEMS Microbiology Letters 183. 81-88;
2. Bowman, J.P., Rea, S.M., McCammon, S.A., McMeekin, T.A. (2000) Diversity and community structure within anoxic sediment from marine salinity meromictic lakes and a coastal meromictic marine ... basin, Vestfold Hills, Eastern Antarctica. Environmental Microbiology 2. 227-238;
3. Bowman, J.P., Rea, S.M., Brown, M.V., McCammon, S.A., Smith, M.C., McMeekin, T.A. (2000) Community structure and psychrophily in Antarctic ecosystems Bell, C.R., Brylinsky, M., Johnson-Green, P. (ed.) Microbial Biosystems: New Frontiers, Proceedings of the 8th International Symposium on Microbial Ecology, Halifax, Canada I. 287-291;
4. Bowman, J.P., McCuaig, R.D. (2003) Biodiversity, Community Structural Shifts, and Biogeography of Prokaryotes within Antarctic Continental Shelf Sediment. Applied and Environmental Microbiology 69(5). 2463-2483;
5. Bowman, J.P., McCammon, S.A., Gibson, J.A.E., Robertson, L., Nichols, P.D. (2003) Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments. Applied and Environmental Microbiology. 69(5). 2778-2462;
6. Brown, M.V., Bowman, J.P. (2001) A molecular phylogenetic survey of sea-ice microbial communities (SIMCO) FEMS Microbiology Ecology (35). 267-275;
7. McCammon, S.A., Bowman, J.P. (2000) The taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum nom. rev., sp. nov. and reclassification of [ Flavobacterium ] salegens as Salegentibacter . International Journal of Systematic and Microbial Evolution 50. 1055-1063;
8. Nichols, D., Bowman, J., Sanderson, K., Mancuso-Nichols, C., Lewis, T., McMeekin, T., Nichols, P.D. (1999) Developments with Antarctic microorganisms: culture collections, bioactivity screening, taxonomy, PUFA production and cold-adapted enzymes Current Opinion in Biotechnology 10. 240-246;
9. Vincent, W.F., Bowman, J.P., Rankin, L.M., McMeekin, T.A. (2000) Phylogenetic diversity of picocyanobacteria in Arctic and Antarctic ecosystems Bell, C.R., Brylinsky, M., Johnson-Green, P. (ed.) Microbial Biosystems: New Frontiers, Proceedings of the 8th International Symposium on Microbial Ecology, Halifax Canada I. 317-322;
