Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.
This work was completed as part of ASAC project 2899 (ASAC_2899).
Genomic data obtained during V3 (2008). This is the primary data portal for genomic metadata, and stands for Community cyber-infrastructure for the Advancement of Microbial Ecology Research and Analysis (CAMERA). All genomic data obtained during this cruise will be curated and posted for public consumption at this site, in addition to being deposited in the major public genomic databases.