The response of sea ice and brine water algae and bacteria to changes in salinity, temperature, light and UVB in Terra Nova BayEntry ID: K043_2006_2008_NZ_4
Abstract: Samples of the bottom ice algae and brine algae (within 30cm of the top of the ice) were subjected to temperature, salinity, light and UVB experiments and analysed with PAM rapid light curves to determine the response of the algae to changes they will encounter during the processes of ice formation at the start of winter and ice melting in summer. Brine algae are likely to be adapted to high light ... and high UVB conditions near the surface of the ice and may have produced higher levels of protective pigments, MAA's, therefore the content of MAA's was analysed. Imaging PAM fluorometry was also used to gain a 3D image of photosynthetic stressors during the process of ice freeze and thaw on both algae and bacterial cells. The difference in thylakoid structure with increase in temperature was also analysed. Bacterial cells were collected at the end of exposure and a CTC assay was performed on them to determine the effect of UVB radiation. Bottom ice algae exposed to different light levels and UV exposure was analysed for cell counts, antioxidants and MAAs. Bacterial response to changes in light and salinity was examined by adding a fluorescent stain (CTC) to a melted entire sea ice core, a brine sample and a bottom ice sample and exposing the samples to various combinations of light and salinity. Samples were examined for cells that fluoresce due to metabolized CTC. The bacterial response to light and the presence/absence/inhibition of microalgae was examined using the vital stain and probe technique after exposing melted ice core samples to a range of irradiances for 4 hours. Samples of the brine community were collected and incubated for 5 days to determine the longer term response to light. Tritiated leucine was used calibrate the use of the CTC method.
Start Date: 2006-11-03Stop Date: 2006-12-09
Start Date: 2007-11-10Stop Date: 2007-12-10
TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CHLOROPHYLL
BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
Quality Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigators. Please contact Dr Ken Ryan for more information.
Data Set Progress
Phone: +64 4 463 6083
Fax: +64 4 463 5331
Email: ken.ryan at vuw.ac.nz
Victoria University of Wellington School of Biological Sciences PO BOX 600
Postal Code: 6140
Country: New Zealand
Role: DIF AUTHOR
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Antarctica New Zealand Private Bag 4745
Country: New Zealand
Martin, A., Hall, J.A., Ryan, K.G. 2009. Low salinity and high-level UV-B radiation reduce single-cell activity in Antarctic sea ice bacteria. Applied and environmental microbiology 75: 7570-7573 2009. doi:10.1128/AEM.00829-09
Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177
Ryan, K.G., Cowie, R.O.M, Liggins, E., McNaughtan, D., Martin, A., Davey, S.K. 2009. The short-term effect of irradiance on the photosynthetic properties of Antarctic fast-ice microalgal communities. Journal of phycology. 10.1111/j.1529-8817.2009.00764.x
Koh, E. Biodiversity of prokaryotes in Antarctic sea ice. PhD thesis in progress, Victoria University of Wellington, New Zealand.
Rajanahally, M. Ecophysiology of Antarctic sea ice algae. . PhD thesis in progress, Victoria University of Wellington, New Zealand.
Ryan, K.G., Liggins, E., McNaughtan, D., Martin, A., Tay, M. & Davy S.K. (2008). Imaging PAM visualisation of sea ice algae and their response to environmental change. In: Rudenko, N.; Klepikov, A. (Eds.) SCAR/IASC IPY Open Science Conference “Polar Research - Arctic and Antarctic perspectives in the International Polar Year”: Abstract volume (pp. 264). State Scientific Center of the Russian Federation the Arctic and Antarctic Research Institute, Elektronsdart-Print Joint-Stock Company. ISBN 978-5-98364-013-09
Rajanahally, M, Bryant, L. Koh, EY, Davy S., Martin A, Ryan KG (2008) Antarctic sea ice productivity and ecophysiology. NZ Marine Sciences Society Conference Christchurch July 2008.
Rajanahally, M, Ryan KG and Davy S (2007). Antarctic microalgae: physiological responses to environmental changes. New Zealand Marine Sciences Society Annual Conference; Hamilton, New Zealand.
Cowie, R., Liggins L., McNaughtan D, Martin A, Davy S., and Ryan KG (2008) The effect of irradiance on Antarctic fast-ice microalgal communities Antarctic New Zealand Annual Conference, Dunedin July 2008.
Rajanahally, M, Bryant, L. Koh, EY, Davy S., Martin A, Ryan KG (2008). Antarctic sea ice productivity and ecophysiology. Antarctic New Zealand Annual Conference, Dunedin July 2008.
Ryan KG, Davy, SK, O’Toole, R, Martin, A, Ralph P (2006) Effects of Salinity and Light on the Microbial Community in Antarctic Sea Ice. SCAR XXIX/ COMNAP XVIII Conference Hobart 2006.
Creation and Review Dates
DIF Creation Date: 2005-09-22
Last DIF Revision Date: 2010-02-23