The bacterial biomass of sea ice from Seabees Hook, Cape Hallett region
Entry ID: K043_2003_2005_NZ_2

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Summary
Abstract: A taxonomic assessment of sea ice bacteria abundance and their response to climate induced stress was investigated from the sea ice around Seabee Hook, Cape Hallett. Four sites at different points around Seabee Hook were established for a representative survey of the annual ice present in Edisto Inlet. The ice had broken out the previous year and new ice formed over the winter providing ideal conditions to compare the distribution and abundance of bacteria occurring in pack ice at the tongue of the Mertz Glacier with bacteria from fast ice at Cape Hallett. Bacteria were collected from the ice at regular intervals with ice cores. A complete ice profile was extracted at each site to examine community composition and structure. Water samples were also collected from 5, 25 and 50m depths and processed in the same way as ice cores and compared. In the lab, bacterial samples were grown under light and dark conditions. Epi-fluorescence microscopy was used to analyse the metabolic activity of bacterial cells by CTC staining (which reveals metabolically active cells), DAPI staining (total cell numbers), VSP (Vital Stain and Probe which utilises oligonucleotide probes to distinguish metabolic activity), DVC (Direct Viable Count - which indicates whether cells are capable of undergoing division) and VIAGRAM (which distinguishes between live and dead cells and also gram positive and gram negative cells). The results were examined using laser based technology (flow cytometry) and epi-fluorescence microscopy. If bacteria do respond to light, one possible explanation is that they respond to increased levels of algal photosynthate. Sugars produced by photosynthesis may be released into the seawater and then be taken up by bacterial cells. To test this idea, 14CO2 was fed to sea ice algae and then they were incubated in the light at -2 °C. The algal cells were removed and the filtrate containing labelled photosynthate was fed to sea ice bacteria. Samples were analysed by a Liquid Scintillation counter.

Related URL
Link: GET DATA
Description: Andrew Martin MSc pdf


Link: GET DATA
Description: Andrew Martin PhD pdf


Link: VIEW RELATED INFORMATION
Description: Investigators research page


Geographic Coverage
 N: -72.3166 S: -72.3166  E: 170.2666  W: 170.2666

Temporal Coverage
Start Date: 2003-11-19
Stop Date: 2003-12-16

Start Date: 2004-11-12
Stop Date: 2004-12-07


Location Keywords
CONTINENT > ANTARCTICA
CONTINENT > ANTARCTICA > CAPE HALLETT
CONTINENT > ANTARCTICA > SEABEES HOOK
GEOGRAPHIC REGION > POLAR


Science Keywords
TERRESTRIAL HYDROSPHERE >WATER QUALITY/WATER CHEMISTRY >CHLOROPHYLL    [Definition]
BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE    [Definition]
BIOSPHERE >AQUATIC ECOSYSTEMS >PLANKTON >PHYTOPLANKTON    [Definition]
BIOSPHERE >AQUATIC ECOSYSTEMS >MARINE HABITAT    [Definition]
BIOSPHERE >ECOLOGICAL DYNAMICS >COMMUNITY DYNAMICS >BIODIVERSITY FUNCTIONS    [Definition]
BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >PHOTOSYNTHESIS    [Definition]
BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >BIOMASS DYNAMICS    [Definition]
BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >PRIMARY PRODUCTION    [Definition]


ISO Topic Category
BIOTA
OCEANS


Platform
FIELD SURVEYS    [Information]
LABORATORY    [Information]
FIELD INVESTIGATION    [Information]


Project
LGP >Latitudinal Gradient Project    [Information]
EBA >Evolution and Biodiversity in the Antarctic    [Information]


Quality
The data is contained in the thesis. Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigator. Please contact Dr Ken Ryan for more information.


Data Set Progress
COMPLETE


Data Center
Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand    [Information]
Data Center URL: http://www.antarcticanz.govt.nz/

Data Center Personnel
Name: SHULAMIT GORDON
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand


Personnel
KEN RYAN
Role: INVESTIGATOR
Phone: +64 4 463 6083
Fax: +64 4 463 5331
Email: ken.ryan at vuw.ac.nz
Contact Address:
Victoria University of Wellington
School of Biological Sciences
PO BOX 600
City: Wellington
Postal Code: 6140
Country: New Zealand


SHULAMIT GORDON
Role: DIF AUTHOR
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand


Publications/References
Martin, A. 2005. Antarctic bacteria, sea ice ecosystem dynamics and global climate change. MSc thesis, Victoria University of Wellington, New Zealand.

Martin AR, Hall J, O’Toole R, Davy SK, Ryan KG, (2008). High single-cell metabolic activity in Antarctic sea ice bacteria. Aquatic Microbial Ecology 52, 25-31.

Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177

Creation and Review Dates
DIF Creation Date: 2005-09-22
Last DIF Revision Date: 2010-02-23

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