The bacterial biomass of sea ice from Seabees Hook, Cape Hallett regionEntry ID: K043_2003_2005_NZ_2
Abstract: A taxonomic assessment of sea ice bacteria abundance and their response to climate induced stress was investigated from the sea ice around Seabee Hook, Cape Hallett. Four sites at different points around Seabee Hook were established for a representative survey of the annual ice present in Edisto Inlet. The ice had broken out the previous year and new ice formed over the winter providing ideal ... conditions to compare the distribution and abundance of bacteria occurring in pack ice at the tongue of the Mertz Glacier with bacteria from fast ice at Cape Hallett. Bacteria were collected from the ice at regular intervals with ice cores. A complete ice profile was extracted at each site to examine community composition and structure. Water samples were also collected from 5, 25 and 50m depths and processed in the same way as ice cores and compared. In the lab, bacterial samples were grown under light and dark conditions. Epi-fluorescence microscopy was used to analyse the metabolic activity of bacterial cells by CTC staining (which reveals metabolically active cells), DAPI staining (total cell numbers), VSP (Vital Stain and Probe which utilises oligonucleotide probes to distinguish metabolic activity), DVC (Direct Viable Count - which indicates whether cells are capable of undergoing division) and VIAGRAM (which distinguishes between live and dead cells and also gram positive and gram negative cells). The results were examined using laser based technology (flow cytometry) and epi-fluorescence microscopy. If bacteria do respond to light, one possible explanation is that they respond to increased levels of algal photosynthate. Sugars produced by photosynthesis may be released into the seawater and then be taken up by bacterial cells. To test this idea, 14CO2 was fed to sea ice algae and then they were incubated in the light at -2 °C. The algal cells were removed and the filtrate containing labelled photosynthate was fed to sea ice bacteria. Samples were analysed by a Liquid Scintillation counter.
Start Date: 2003-11-19Stop Date: 2003-12-16
Start Date: 2004-11-12Stop Date: 2004-12-07
TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CHLOROPHYLL
BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
Quality The data is contained in the thesis. Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigator. Please contact Dr Ken Ryan for more information.
Data Set Progress
Phone: +64 4 463 6083
Fax: +64 4 463 5331
Email: ken.ryan at vuw.ac.nz
Victoria University of Wellington School of Biological Sciences PO BOX 600
Postal Code: 6140
Country: New Zealand
Role: DIF AUTHOR
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Antarctica New Zealand Private Bag 4745
Country: New Zealand
Martin, A. 2005. Antarctic bacteria, sea ice ecosystem dynamics and global climate change. MSc thesis, Victoria University of Wellington, New Zealand.
Martin AR, Hall J, O’Toole R, Davy SK, Ryan KG, (2008). High single-cell metabolic activity in Antarctic sea ice bacteria. Aquatic Microbial Ecology 52, 25-31.
Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177
Creation and Review Dates
DIF Creation Date: 2005-09-22
Last DIF Revision Date: 2010-02-23