[Parameters: Topic='BIOSPHERE', Term='AQUATIC ECOSYSTEMS', Variable_Level_1='MARINE HABITAT']
Investigation of the role of anti-freeze glyco-proteins in the circulation system of Antarctic fishEntry ID: K014_2009_2010_NZ_1
Abstract: In order to investigate how Antarctic fish deal with ice in their blood circulatory system, Trematomus bernacchii and Pagothenia borchgrevinki Antarctic fish were captured for experimental investigation. A variety of biochemical techniques were used to isolate Anti-Freeze Glyco-Proteins (AFGPs) from Antarctic fish blood, which was then conjugated directly to a fluorescent marker. We synthesised ... silicon dioxide nanobeads incorporating a fluorescent probe and coupled these to AFGP via a multimeric linking protein or dendrimer based on G4.5 poly(amidoamine). These modified nanobeads, consisting of a fluorescent particle surrounded by AFGPs, were designed to serve as a proxy for ice crystals that have bound AFGPs. When introduced into the circulation of the fish, we were able to follow the fate of free fluorescent AFGP and particle-bound fluorescent AFGP (the latter serving as a proxy for ice in the circulation). Frozen and alcohol-fixed tissues were returned to New Zealand for further laboratory analysis.
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Horizontal Resolution Range: 30 meters - < 100 meters
Creation and Review Dates
Last DIF Revision Date: 2008-07-18